Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cationic nanotrap curbs UVB-induced cutaneous photodamage via exosomal cfNA capture.

Biomaterials·2026
Same author

A dual-mode electrochemical-colorimetric aptasensor based on HCR-CRISPR/Cas12a cascade amplification for MTGase detection.

Food research international (Ottawa, Ont.)·2026
Same author

Upconversion Nanoparticle-Guided Virtual Deformable Mirror for Computational Adaptive Optics in Scattering Fluorescence Microscopy.

Nano letters·2026
Same author

Pore size-regulated MOF nanozyme-based colorimetric sensor array for rapid detection and classification of mycotoxins.

Biosensors & bioelectronics·2026
Same author

Single-granule profiling reveals that RNP granule pH marks cellular translation.

iScience·2026
Same author

A Portable Microfluidic Platform Based on Gravity-Mediated Magnetic Control for On-Site DNA Extraction and Multiplexed Detection of Foodborne Pathogens.

Analytical chemistry·2026

Related Experiment Video

Updated: Jan 17, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.3K

Deep learning enhanced dual-mode fluorescence cooperative imaging using upconversion nanoparticles.

Shijia Wu, Xiao Zhou, Weilong Kong

    Optics Express
    |September 23, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Deep learning enhances multiphoton microscopy (MPM) for deep-tissue imaging. This novel dual-modal approach overcomes resolution-penetration trade-offs, enabling clearer visualization in scattering tissues.

    More Related Videos

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.2K

    Related Experiment Videos

    Last Updated: Jan 17, 2026

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
    07:13

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

    Published on: May 16, 2022

    2.3K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.2K

    Area of Science:

    • Biomedical Optics
    • Advanced Imaging Techniques
    • Nanotechnology

    Background:

    • Multiphoton microscopy (MPM) excels in deep-tissue imaging but faces challenges with light scattering and resolution-penetration trade-offs.
    • Achieving high-resolution imaging at significant depths in biological tissues remains a critical limitation.

    Purpose of the Study:

    • To develop a deep learning-enhanced dual-modal fluorescence cooperative imaging (DL-DMFC) approach for high-resolution deep-tissue imaging.
    • To overcome the inherent resolution-penetration trade-off in conventional MPM.

    Main Methods:

    • Utilized lanthanide upconversion nanoparticles (UCNPs) to simultaneously generate two-photon (higher penetration) and four-photon (higher resolution) fluorescence under a single excitation source.
    • Employed artificial neural networks with adversarial training and cyclic consistency constraints for cross-domain mapping of dual-modal signals.
    • Developed a synergistic excitation and computational framework for enhanced imaging.

    Main Results:

    • Achieved high-resolution, anti-scattered 3D imaging beyond 500 μm depth.
    • Demonstrated a 51% enhancement in transverse resolution.
    • Successfully addressed the penetration-resolution trade-off in MPM.

    Conclusions:

    • The DL-DMFC approach provides a novel strategy for deep-tissue thick scattering imaging.
    • This method significantly improves imaging depth and resolution in MPM.
    • Offers a new pathway for advanced biological and medical imaging applications.