Related Experiment Video

Updated: Aug 2, 2026

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.7K

Protective Effect of Conditioned Medium from Bone Marrow Mesenchymal Stem Cells on the Cornea of Rabbits with

V V Tiulina1, S V Lavrushkina1, A I Dovgii2

  • 1A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

Bulletin of Experimental Biology and Medicine
|September 25, 2025
PubMed

Insights

Bone marrow mesenchymal stem cell secretome effectively protected rabbit corneas from dry eye damage. This treatment suppressed MMP-9 activation, suggesting a potential therapy for dry eye syndrome.

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Cell Biology

Background:

  • Dry eye syndrome (DES) is a prevalent condition causing corneal damage.
  • Impaired blinking reflex can exacerbate DES and lead to epithelial barrier dysfunction.
  • Matrix metalloproteinase-9 (MMP-9) activation contributes to extracellular matrix degradation in the cornea.

Purpose of the Study:

  • To evaluate the efficacy of bone marrow mesenchymal stem cell (BMMSC) secretome in preventing corneal damage in a rabbit model of DES.
  • To investigate the effect of BMMSC secretome on MMP-9 activation in the tear film.

Main Methods:

  • An experimental model of dry eye syndrome was induced in rabbits by impairing the blinking reflex.
  • Rabbits were treated with a conditioned medium containing the secretome of BMMSCs.
  • Corneal epithelial protection and MMP-9 activation in the tear film were assessed.

Main Results:

  • The BMMSC secretome demonstrated a significant protective effect on the corneal epithelial layer.
  • Treatment with BMMSC secretome suppressed the activation of MMP-9 in the tear film.
  • These findings suggest that BMMSC secretome can mitigate corneal damage associated with DES.

Conclusions:

  • Bone marrow mesenchymal stem cell secretome shows therapeutic potential for treating dry eye syndrome.
  • BMMSC secretome may act by inhibiting MMP-9 activity, thereby preserving corneal epithelial barrier function.
  • Further research into BMMSC secretome as a treatment for ocular surface diseases is warranted.
Keywords:
CorneaDry eye syndromeMatrix metalloproteinase-9Stem cell secretomeTear film

More Related Videos

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium07:10

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

Published on: April 6, 2022

4.4K
Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration07:39

Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration

Published on: August 4, 2023

2.6K

Related Concept Videos

Anatomy of the Eyeball01:20

Anatomy of the Eyeball

The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...
Microbiome of the Eye01:22

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...