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Published on: February 17, 2023
From form to function: New heights in kidney organoids
Jonathan Levinsohn1, Katalin Susztak2
1Renal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA; Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA; Penn/CHOP Kidney Innovation Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Genetics, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Pediatrics, Division of Nephrology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Researchers created kidney organoids using region-specific progenitor assembloids that mimic native kidney architecture. These organoids show functional and disease modeling capabilities after transplantation in mice.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Organoid development
Background:
- Organoids offer promising models for studying organ development and disease.
- Current kidney organoid models struggle to replicate the complex spatial patterning and function of native kidneys.
Purpose of the Study:
- To develop novel kidney organoids that better mimic native kidney spatial patterning and function.
- To create region-specific progenitor assembloids for improved kidney organoid models.
Main Methods:
- Development of region-specific progenitor assembloids.
- Transplantation of assembloids into mice to promote differentiation.
- Assessment of functional and disease modeling capabilities.
Main Results:
- Successfully generated kidney assembloids with improved spatial patterning.
- Demonstrated enhanced differentiation and functional maturation after in vivo transplantation.
- Validated the utility of these organoids for disease modeling.
Conclusions:
- Region-specific progenitor assembloids represent a significant advancement in kidney organoid technology.
- These enhanced organoids hold great potential for kidney disease research and drug screening.
Related Concept Videos
01:45Kidney Structure
01:28Kidney Transplant I: Introduction
01:12Internal Anatomy of the Kidney
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...