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Simple, Affordable, and Modular Patterning of Cells using DNA
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Programmable DNA shell scaffolds for directional membrane budding.

Michael T Pinner1,2, Hendrik Dietz3,4

  • 1Laboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Garching bei München, Germany.

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|October 9, 2025
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Summary
This summary is machine-generated.

Researchers mimicked virus assembly using DNA origami to control cellular membrane budding. This DNA-based system creates vesicles with DNA shells, replicating natural cell processes for drug delivery and synthetic biology.

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Area of Science:

  • Nanotechnology
  • Synthetic Biology
  • Biophysics

Background:

  • Controlling cellular membrane dynamics is crucial for replicating biological processes at the nanoscale.
  • Understanding membrane budding is key to endocytic and exocytic pathways.

Purpose of the Study:

  • To develop a system mimicking virus assembly for controlled directional membrane budding.
  • To utilize DNA origami for precise control over vesicle formation and DNA shell encapsulation.

Main Methods:

  • Employing three-dimensional DNA origami to create cholesterol-modified triangles.
  • Self-assembly of DNA triangles into polyhedral shells on lipid vesicles.
  • Strategic cholesterol positioning to direct bud growth and vesicle formation.

Main Results:

  • Achieved controlled directional membrane budding and spontaneous neck scission.
  • Produced daughter vesicles with DNA endo- or exoskeletons, resembling clathrin-coated vesicles.
  • Demonstrated rapid kinetics and compatibility with various lipid compositions.
  • Created nested bivesicular objects with encapsulated DNA shells.

Conclusions:

  • The DNA origami system effectively mimics viral assembly to control membrane budding.
  • This approach replicates key aspects of natural endocytic and exocytic pathways.
  • Opens new avenues for membrane mechanics research, targeted drug delivery, and synthetic biology applications.