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Related Experiment Video

Updated: Jan 15, 2026

Generation of Functional Endodermal Hepatic Organoids
08:45

Generation of Functional Endodermal Hepatic Organoids

Published on: May 2, 2025

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Protocol for generating liver organoids containing Kupffer cells using human iPSCs.

Yang Li1, Yunzhong Nie2, Hideki Taniguchi2

  • 1Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.

STAR Protocols
|October 11, 2025
PubMed
Summary

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Oxygenated Perfusion Enhances Hepatocyte Function in Human iPSC-liver Tissue.

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Effect of the intravenous acetaminophen clinical pathway on postoperative analgesia in spinal fusion surgery.

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Human pluripotent stem cell-derived fetal hepatic stellate cells promote vascularization and maturation in liver organoids.

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CORRIGENDUM TO "EFFICACY AND SAFETY OF A DEXAMETHASONE-BASED MOUTHWASH TO PREVENT CHEMOTHERAPY-INDUCED STOMATITIS IN WOMEN WITH BREAST CANCER: A MULTICENTRE, OPEN-LABEL, RANDOMISED PHASE 2 STUDY" [J EVID BASED DENT PRACT. 2023 SEP;23(3):101896].

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This study introduces a protocol to create advanced liver organoids by incorporating Kupffer cells (liver-resident macrophages) derived from human stem cells. These Kupffer cell-liver organoids (KuLOs) offer a new model for studying liver immunity and disease.

Area of Science:

  • Stem cell biology
  • Immunology
  • Hepatology

Background:

  • Accurate recapitulation of native tissue physiology in vitro requires the integration of resident immune cells.
  • Liver organoids are valuable models for studying liver function and disease.

Purpose of the Study:

  • To present a protocol for integrating Kupffer cells into liver organoid models.
  • To establish a platform for investigating Kupffer cell roles in liver development and disease.

Main Methods:

  • Generating Kupffer cell progenitors and hepatic endoderm from human induced pluripotent stem cells (iPSCs).
  • Establishing liver organoids containing Kupffer cells (KuLOs).

Main Results:

  • Successful integration of Kupffer cells into iPSC-derived liver organoids.
Keywords:
cell Biologycell differentiationorganoids

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Last Updated: Jan 15, 2026

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  • Development of a detailed protocol for creating Kupffer cell-liver organoids (KuLOs).

    • Conclusions:

    • The developed protocol provides a novel platform for studying Kupffer cell functions in a controlled in vitro setting.
    • KuLOs can advance research into liver development, immunity, and disease pathogenesis.