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A viscosity-activated and tumor-specific NIR probe for precise imaging of cancer cells

Xue Wu1, Lingling Cao1, Qiuyue Zhao1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, People's Republic of China. 1031208296@qq.com.

Chemical Communications (Cambridge, England)
|October 20, 2025
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.