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Electron fourier ptychography for phase reconstruction.

Jingjing Zhao1, Chen Huang2, Ali Mostaed2

  • 1The Rosalind Franklin Institute, Didcot, OX11 0QX, UK. jingjing.zhao@rfi.ac.uk.

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|October 31, 2025
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Summary

Electron Fourier ptychography enables high-resolution phase reconstruction for transmission electron microscopy. This accessible method works for various materials without instrumental changes.

Keywords:
Cryogenic conditionElectron Fourier ptychographyLow fluencePhase reconstruction

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Area of Science:

  • Materials Science
  • Microscopy
  • Crystallography

Background:

  • Exit wavefunction reconstruction is crucial for structural analysis in transmission electron microscopy (TEM).
  • Existing methods may face limitations with beam-sensitive or radiation-resistant materials.

Purpose of the Study:

  • To introduce and demonstrate electron Fourier ptychography for high-resolution phase reconstruction.
  • To provide an accessible alternative for structural studies using TEM.

Main Methods:

  • Utilized electron Fourier ptychography with a modified iterative phase retrieval algorithm.
  • Collected data in an alternative optical geometry.
  • Applied the method to both radiation-resistant and beam-sensitive materials, including Cry11Aa protein crystals.

Main Results:

  • Achieved high-resolution phase reconstruction of the exit wave.
  • Demonstrated a spatial resolution of 0.63 nm at a fluence of 4.5 × 10^2 e-/nm^2.
  • Validated the method on Cry11Aa protein crystals under cryogenic conditions.

Conclusions:

  • Electron Fourier ptychography offers a broadly accessible approach for structural studies.
  • The method requires no instrumental modifications and integrates seamlessly with existing software.
  • Successfully applied to reconstruct phase information for diverse materials.