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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Assembly of Cytoskeletal Filaments01:18

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Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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Enzyme-Instructed Self-Assembly for Cellular Supramolecular Chemistry.

Yali Huang1, Xingjie Hu2, Zhimou Yang2

  • 1Department of Chemistry, Brandeis University, Waltham, USA.

Chemistry, an Asian Journal
|November 14, 2025
PubMed
Summary
This summary is machine-generated.

Enzyme-instructed self-assembly (EISA) uses enzymes to create nanostructures within cells, offering precise control over assembly. This catalytic approach advances biomaterials and therapeutics by mimicking biological systems.

Keywords:
biologyenzymegrowth factormedicineself‐assembly

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Area of Science:

  • Supramolecular Chemistry
  • Chemical Biology
  • Nanotechnology

Background:

  • Enzyme-instructed self-assembly (EISA) leverages endogenous enzymatic activity for controlled nanostructure formation.
  • Unlike pH, redox, or light triggers, EISA utilizes enzyme localization and kinetics for precise spatiotemporal control.
  • Existing reviews cover EISA mechanisms and applications, but a conceptual framework for supramolecular chemical biology is needed.

Purpose of the Study:

  • To position EISA as a framework for supramolecular chemical biology.
  • To emphasize EISA's role in mimicking protein assemblies and bridging molecular design with cellular function.
  • To explore EISA's potential beyond alkaline phosphatases to multi-enzyme networks for advanced applications.

Main Methods:

  • Conceptual analysis and perspective on EISA's capabilities.
  • Discussion of EISA's programmability for conformational and morphological switching.
  • Exploration of in situ formation of supramolecular architectures within cellular environments.

Main Results:

  • EISA enables programmable switching and creation of growth factor-mimicking assemblies.
  • Artificial supramolecular architectures can be formed in situ inside or around cells.
  • EISA acts as a catalytic strategy for constructing functional supramolecular systems in vivo.

Conclusions:

  • EISA offers a new direction for integrating enzymatic control with nanoscale self-organization in cellular supramolecular chemistry.
  • Generalizing EISA to programmable multi-enzyme networks advances adaptive biomaterials, programmable therapeutics, and synthetic cellular machines.
  • EISA provides precise, context-dependent control over nanostructure formation within biological systems.