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Updated: Jan 11, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
Structure and gene cluster of the capsular polysaccharide of Acinetobacter baumannii K104
Anna M Shpirt1, Zakhar V Serpokrylov1, Andrei V Perepelov1
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
Structure of the Acinetobacter baumannii MAR20-4513 (K104) capsular polysaccharide (CPS) was established using Smith degradation, 1H and 13C NMR spectroscopy, including two-dimensional homonuclear 1Н,1Н COSY, TOCSY, ROESY and heteronuclear 1Н,13C HSQC and HMBC experiments, and modeling. The K104 CPS is composed of a branched pentasaccharide K unit containing two d-Glcp residues, and one residue each of d-GalpNAc, d-GlcpNAc and d-Galp. The following structure of the pentasaccharide repeating unit of the CPS was established. Functions of genes in the K locus of A. baumannii K104 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.
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