Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially and Chemically Specific Optical Control of Cells via Supervised and Automated Target Selection.

ACS photonics·2026
Same author

Crosstalk-Free Real-Time Precision Opto-Control of Biochemical Processes Through Intra-Pixel Optical Decoupling.

Chemistry methods : new approaches to solving problems in chemistry·2026
Same author

Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.

Analytical chemistry·2026
Same author

Spontaneous or Stimulated? Investigating Raman's Detection Limits in Aqueous Environments.

The journal of physical chemistry. B·2025
Same author

Real-Time and Site-Specific Perturbation of Dynamic Subcellular Compartments Using Femtosecond Pulses.

Small science·2025
Same author

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy.

Analytical chemistry·2025

Related Experiment Video

Updated: Jan 10, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K

Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.

Mingmin Zhou1, Bin Dong1,2, Laura Lukov1

  • 1James Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, IN 47907, USA.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

Homodyne epi-coherent anti-Stokes Raman scattering (epi-CARS) microscopy offers label-free chemical imaging of cell adhesion dynamics. This technique precisely visualizes cell-substrate interactions and lipid droplets, advancing cell migration studies.

Keywords:
Coherent anti-Stokes Raman scatteringcell adhesioninterfaceinterferencemitosis

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.7K
Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

3.5K

Related Experiment Videos

Last Updated: Jan 10, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.7K
Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

3.5K

Area of Science:

  • Biophysics
  • Cell Biology
  • Chemical Imaging

Background:

  • Understanding cell adhesion is crucial for cell migration and extracellular matrix interactions.
  • Conventional microscopy methods lack chemical specificity or require fluorescent labels for interfacial studies.

Purpose of the Study:

  • To develop a label-free microscopy technique for studying cell adhesion dynamics at interfaces.
  • To investigate the precise visualization of cell-substrate interactions and lipid droplets using epi-CARS.

Main Methods:

  • Utilized homodyne epi-coherent anti-Stokes Raman scattering (epi-CARS) microscopy focused at interfaces.
  • Incorporated a pinhole in a confocal epi-CARS system to reject out-of-focus reflections.
  • Applied the method to study cell-substrate adhesion dynamics in mitotic and post-mitotic cells.

Main Results:

  • Epi-CARS demonstrated intrinsic interfacial selectivity with negative contrast at attachment areas, precisely measuring cell-substrate distances.
  • The technique provided high contrast for cellular lipid droplets in an inverted microscope configuration.
  • Observed adhesion site splitting during mitosis and outward development during postmitotic spreading.
  • Characterized distinct adhesion patterns and water layer thicknesses at leading and retracting cell edges post-mitosis.

Conclusions:

  • Homodyne epi-CARS microscopy is a powerful label-free tool for interfacial cell biology.
  • The method enables precise nanoscale imaging of cell adhesion and lipid dynamics.
  • Revealed dynamic changes in cell adhesion during mitosis and spreading with unprecedented detail.