Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Optical Control of Living Cells Electrical Activity by Conjugated Polymers
10:16

Optical Control of Living Cells Electrical Activity by Conjugated Polymers

Published on: January 28, 2016

Spatially and Chemically Specific Optical Control of Cells via Supervised and Automated Target Selection.

Bin Dong1, R Michael Everly2, Shivam Mahapatra2

  • 1James Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States; Purdue Institute for Cancer Research, West Lafayette, Indiana 47907, United States.

ACS Photonics
|June 26, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crosstalk-Free Real-Time Precision Opto-Control of Biochemical Processes Through Intra-Pixel Optical Decoupling.

Chemistry methods : new approaches to solving problems in chemistry·2026
Same author

Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.

Analytical chemistry·2026
Same author

Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.

bioRxiv : the preprint server for biology·2025
Same author

Spontaneous or Stimulated? Investigating Raman's Detection Limits in Aqueous Environments.

The journal of physical chemistry. B·2025
Same author

Real-Time and Site-Specific Perturbation of Dynamic Subcellular Compartments Using Femtosecond Pulses.

Small science·2025
Same author

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy.

Analytical chemistry·2025

We developed a software-assisted real-time precision opto-control (S-RPOC) system for enhanced biological control. This innovative optical method precisely regulates cell functions in real time, overcoming limitations of traditional chemical interventions.

Area of Science:

  • Cell biology
  • Biophysics
  • Optical engineering

Background:

  • Traditional chemical interventions for cell function regulation lack spatial and temporal precision.
  • Existing optical control methods face limitations in accuracy, response time, and flexibility.

Purpose of the Study:

  • To develop a novel system for precise, real-time optical control of biological functions.
  • To overcome the limitations of current optogenetic and photoperturbation techniques.

Main Methods:

  • Developed a software-assisted real-time precision opto-control (S-RPOC) system.
  • Synchronized galvo scanning, user input, chemical detection, decision-making, and laser activation.
  • Enabled automated target selection and flexible user input for diverse optical treatments.
Keywords:
RPOCmitochondrionoptical controlphotoswitchablereactive oxygen speciesreal-time

More Related Videos

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Related Experiment Videos

Last Updated: Jun 27, 2026

Optical Control of Living Cells Electrical Activity by Conjugated Polymers
10:16

Optical Control of Living Cells Electrical Activity by Conjugated Polymers

Published on: January 28, 2016

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Main Results:

  • Demonstrated selective organelle photoperturbation.
  • Achieved targeted compound activation within specific cells.
  • Controlled cell division through centrosome perturbation.
  • Performed label-free blue light treatment of cells.

Conclusions:

  • S-RPOC system offers advanced capabilities for precise, real-time control of cellular functions.
  • The system facilitates site-specific investigation of biomolecular activities.
  • S-RPOC provides improved approaches for managing biological sample functions and responses.