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Updated: Jan 9, 2026

Studying TGF-&#946; Signaling and TGF-&#946;-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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Human Fidgetin Modulates Cell Migration and EMT in Breast Cancer Cells.

Suranjana Mukherjee1, Jyotirmoy Chatterjee1

  • 1School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, India.

Cell Biology International
|December 5, 2025
PubMed
Summary
This summary is machine-generated.

Depleting fidgetin, a microtubule enzyme, reduces cancer cell migration and epithelial-mesenchymal transition (EMT) by affecting key transcription factors and microtubule dynamics. These findings highlight fidgetin as a potential therapeutic target for cancer metastasis.

Keywords:
breast cancercell migrationepithelial–mesenchymal transitionfidgetinfocal adhesionsmicrotubule

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Epithelial-mesenchymal transition (EMT) and cell migration are crucial for development and cancer metastasis.
  • Microtubule cytoskeleton and associated proteins are implicated in these cellular processes.

Purpose of the Study:

  • To investigate the role of fidgetin, a microtubule-severing enzyme, in EMT and cell migration.
  • To determine fidgetin's impact on EMT-related transcription factors and microtubule dynamics in breast cancer cells.

Main Methods:

  • Depletion of fidgetin in MDA-MB-231 breast cancer cells.
  • Wound-healing and single-cell motility assays.
  • Analysis of EMT transcription factors (Snail, Slug, Twist, Zeb1), N-cadherin, vimentin, CLIP-170, EB1, and focal adhesions.

Main Results:

  • Fidgetin depletion reduced cell migration rates and expression of Slug and Zeb1.
  • Downregulation of N-cadherin and vimentin was observed in fidgetin-depleted cells.
  • Fidgetin affects microtubule plus-end tracking proteins (+TIPs), reducing EB1 comet area and increasing focal adhesion size.

Conclusions:

  • Fidgetin regulates cancer cell motility by influencing EMT transcription factors and microtubule dynamics.
  • Fidgetin depletion alters focal adhesion size and EB1 protein accumulation.
  • Fidgetin represents a potential therapeutic target for inhibiting cancer metastasis.