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Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...

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Related Experiment Video

Updated: May 8, 2026

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
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Comprehensive Transcriptomic and Epitranscriptomic Profiling of Hepatitis B Virus Transcripts in Two Hepatocellular

Qinan Zhang1,2, Bohan Zhang2, Lei Wang1

  • 1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Genes
|December 30, 2025
PubMed
Summary
This summary is machine-generated.

This study reveals new details about hepatitis B virus (HBV) RNA processing in liver cancer cells, identifying novel splice variants and RNA modifications. Findings help select better models for studying HBV and developing antiviral therapies.

Keywords:
RNA modificationsalternative splicingdirect RNA sequencinghepatitis B viruspolyadenylationtranscriptome

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Area of Science:

  • Virology
  • Molecular Biology
  • Hepatology

Background:

  • Hepatitis B virus (HBV) post-transcriptional regulation and epitranscriptomic modifications are not fully understood.
  • Characterizing HBV's RNA landscape is crucial for understanding infection and developing treatments.

Purpose of the Study:

  • To systematically profile the transcriptomic complexity and RNA modification landscape of HBV.
  • To analyze HBV's complete transcriptome in hepatocellular carcinoma (HCC) models.

Main Methods:

  • Transfection of HCC cell lines (PLC/PRF/5 and Huh7) with HBV replicon plasmid.
  • Nanopore direct RNA sequencing of total nucleic acids.
  • Bioinformatic analysis of alternative splicing, polyadenylation, and RNA modifications.

Main Results:

  • Identified 34 distinct HBV splice variants, including 14 novel isoforms, with cell-type-specific expression.
  • Detected 30 high-confidence RNA modification sites on HBV transcripts, with 93% conservation.
  • Observed significant heterogeneity in poly(A) tail length distributions between cells.

Conclusions:

  • PLC/PRF/5 cells may better mimic HBV immune evasion, while Huh7 cells show efficient replication due to longer poly(A) tails.
  • Findings provide a benchmark for selecting appropriate HBV research models.
  • Identified transcriptomic features offer potential targets for novel antiviral strategies.