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RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Published on: February 13, 2013
Identification of upregulated genes in pulmonary hypertension using RNA sequencing profiling
Jino Blessy J1, Jayasurya R1, Shanmugapriya Murugan1
1Department of Bioinformatics, Sri Ramachandra Faculty of Engineering and Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, India.
None:
Pulmonary hypertension (PH) is a chronic and progressive disease that is characterized by increased pulmonary arterial blood pressure, causing heart strain and eventual heart failure. In the current study PH RNA sequencing (RNA-Seq) dataset involving 8 patients, consisting of 4 cases and 4 controls was selected for analysis. Pathway analysis revealed the involvement of the key genes in pathways crucial for PH. The protein-protein interaction (PPI) network analysis using the STRING database identified key hub genes that were significantly upregulated, including HAUS4, TUBB4A, TUBG1, NEED1, SASS6, NIN, DCUN1D3, CCDC22, ATP2B2 and LRRC37A. These hub gene-encoded proteins can be prominent drug targets for future interventions aimed at treating pulmonary hypertension.
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