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The nuclear cap-binding complex regulates subcellular RNA processing and surveillance of coding and noncoding RNAs.

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  • 1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, United States.

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|January 7, 2026
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This summary is machine-generated.

This study reveals diverse RNA cleavage events in plant cell nuclei and cytoplasm, uncovering new roles for the nuclear cap-binding complex in RNA processing and surveillance.

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Genomics

Background:

  • RNA cleavage is crucial for RNA processing and regulation.
  • Existing methods for profiling RNA cleavage are limited, primarily focusing on cytoplasmic events and neglecting subcellular differences.

Purpose of the Study:

  • To investigate genome-wide RNA cleavage events within different cellular compartments (nucleoplasm and cytoplasm) in Arabidopsis thaliana.
  • To identify novel RNA cleavage and processing events occurring in the plant nucleus.

Main Methods:

  • Coupling subcellular RNA fractionation with degradome profiling.
  • Analyzing RNA cleavage patterns in isolated nucleoplasmic and cytoplasmic fractions.

Main Results:

  • Messenger RNA (mRNA) cleavage was predominant in cytoplasmic fractions.
  • A wide range of nucleoplasm-enriched RNA cleavage events were identified, including pre-mRNA cleavage and processing of noncoding RNAs (microRNAs, ribosomal RNAs, small nucleolar RNAs, enhancer-associated RNAs, retrotransposon-derived RNA).
  • CAP-BINDING PROTEIN80/ABA HYPERSENSITIVE1 was implicated in mRNA surveillance in the perinuclear cytoplasm and stabilization of nucleoplasmic RNAs.

Conclusions:

  • RNA cleavage is highly compartmentalized within plant cells, with distinct events occurring in the nucleus and cytoplasm.
  • The nuclear cap-binding complex plays multifaceted roles in nucleoplasmic RNA processing and cytoplasmic mRNA surveillance.