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Updated: Feb 6, 2026

Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
A review on the impact of green deep eutectic solvents on the stability and activity of cellulase
Madushmita Hatimuria1, C Nikesh Chandran1, Ananya V1
1Department of Chemistry, School of Chemical Sciences, Central University of Karnataka, Kadaganchi, Karnataka, 585367, India.
Abstract:
Enzymes are highly selective biocatalysts widely used in industrial processes due to their efficiency and specificity. Among them, cellulases play a crucial role in the degradation of cellulose, with significant applications across industries such as bioethanol production, textiles, and food processing. However, cellulase activity and stability are often compromised by reaction conditions, limiting their industrial scalability. Traditional aqueous and organic solvents pose challenges related to substrate solubility, toxicity, and environmental safety. Recently, green deep eutectic solvents (DESs) have emerged as promising alternatives that enhance enzyme performance while being cost-effective, biodegradable, and environmentally friendly. This review examines the effects of DESs on cellulase activity and stability, emphasizing their potential as co-solvents to address existing limitations. Polyol-based DESs are shown to enhance and stabilize cellulase activity. However, the concentration of DESs must be carefully optimized to achieve optimal enzyme performance.
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