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Workflow for Gene Overexpression and Phenotypic Characterisation in Taraxacum kok-saghyz
Loredana Lopez1, Michele Antonio Savoia2, Loretta Daddiego1
1Trisaia Research Center, Italian National Agency for New Technologies Energy and Sustainable Economic Development, (ENEA), 75026 Rotondella, MT, Italy.
None:
Taraxacum kok-saghyz (Tks) is a promising plant species for natural rubber (NR) production and represents a model for studying NR biosynthesis in the Asteraceae family. The generation of transgenic plants overexpressing a gene of interest is a well-established strategy to investigate gene function and potential interactions. Here, we present a comprehensive workflow-from the construction of an overexpression vector to the generation, identification, and propagation of stable transgenic Tks lines. In addition, we describe a rapid and reliable method for quantifying NR content in transformed plants, providing essential phenotypic characterisation in this species.
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