[Current progress in reverse genetics technology of parainfluenza virus type 5 and its applications]

Parainfluenza virus type 5 (PIV5) is a negative-sense single-stranded RNA virus belonging to the genus Rubulavirus of the family Paramyxoviridae. The virus has a broad host range and can infect various animals, including ticks, horses, dogs, pigs, hamsters, guinea pigs, cattle, and giant pandas. With extremely low pathogenicity in humans and a favorable safety profile, PIV5 has garnered significant attention as a promising novel vaccine vector platform. This article outlines the pathogenic biological characteristics of PIV5, summarizes the advances in reverse genetics technologies for PIV5 and other common paramyxoviruses, and analyzes the advantages and challenges of PIV5 as a vaccine vector. The aim is to provide insights for the development of novel PIV5-based vector vaccines.