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Updated: Mar 12, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

Comparative Evaluation of Four Multiple Allergen Simultaneous Test (MAST) Systems in Clinical Practice.

Sojin Lee¹, Junhyup Song², Kyung-A Lee¹

¹Department of Laboratory Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.

Journal of Asthma and Allergy

|March 11, 2026

Summary

Four automated multiple allergen simultaneous test (MAST) systems showed high agreement for allergy screening. SGTi-Allergy Screen best matched ImmunoCAP results, while AdvanSure Alloscreen Max108 offered broad coverage, guiding laboratory selection.

Keywords:

MAST allergen-specific IgE allergens automated analyzer hypersensitivity immunoassay immunoglobulin E multiple allergen simultaneous test

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Area of Science:

* Clinical immunology and allergy diagnostics.
* Development and validation of in vitro diagnostic assays.

Background:

* Multiple allergen simultaneous tests (MASTs) are convenient and cost-effective for screening allergen-specific immunoglobulin E.
* New automated MAST analyzers with expanded allergen panels are available in Korea, but comparative data are limited.

Purpose of the Study:

* To evaluate and compare the performance of four automated MAST systems available in Korea.
* To assess the concordance between different MAST platforms and with ImmunoCAP, a reference standard.

Main Methods:

* Retrospective analysis of 200 residual serum samples from patients with suspected allergic diseases.
* Testing performed on AdvanSure Alloscreen, AdvanSure Alloscreen Max108, SGTi-Allergy Screen, and PROTIA Allergy-Q.
* Concordance rates and Cohen's kappa coefficients calculated; discrepant samples re-tested with ImmunoCAP.

Main Results:

* High overall agreement (91-93%) and substantial to almost perfect agreement (κ = 0.67-0.76) among the four MAST systems.
* SGTi-Allergy Screen demonstrated the highest concordance (76.39%) with ImmunoCAP for discrepant samples.
* AdvanSure Alloscreen Max108 showed the highest agreement with its predecessor, AdvanSure Alloscreen.

Conclusions:

* All evaluated MAST systems exhibit substantial to near-perfect qualitative agreement.
* SGTi-Allergy Screen offers superior concordance with the gold standard ImmunoCAP.
* AdvanSure Alloscreen Max108 provides a balance of performance and allergen coverage, suggesting selection should align with laboratory needs.