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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
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Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain
Anne Claire Tangen1, Peyton Harmon2, Anya S Plotnikova3
1Laboratory of Neuropsychology, National Institute of Mental Health, NIH, Bethesda, MD 20892, USA; Neuroscience Institute, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
STAR Protocols
|April 29, 2026
Summary
This study presents a detailed protocol for precise viral microinjections in macaque brains. This method aids in studying neural circuits, development, and diseases in nonhuman primates.
Area of Science:
- Neuroscience
- Primate Research
- Molecular Biology
Background:
- Viral vectors are crucial tools for modifying neurons in nonhuman primates.
- Understanding neural circuits, development, and disease mechanisms requires precise experimental techniques.
Purpose of the Study:
- To provide a comprehensive protocol for stereotactic viral microinjections in the macaque brain.
- To facilitate research into neural circuit organization, function, development, and disease.
Main Methods:
- Detailed steps for pre-surgical imaging and target planning.
- Procedures for performing precise stereotactic viral microinjections.
- Methods for perioperative care, tissue extraction, and slice electrophysiology.
Main Results:
- The protocol enables precise targeting of subcortical and cortical regions in the macaque brain.
- Successful application of viral injections for neuronal modification and subsequent analysis.
Conclusions:
- This protocol offers a standardized and reproducible method for viral vector delivery in macaque brains.
- It supports advanced investigations into the complexities of primate neural systems and related disorders.
