Markers for platelet function testing by flow cytometry: where are we now? A clinical perspective.

Area of Science:

• Hematology
• Clinical Pathology
• Biomedical Engineering

Background:

• Traditional light transmission aggregometry has limitations in platelet function testing.
• Flow cytometry presents an advanced alternative with enhanced capabilities.
• Platelet function analysis is crucial for diagnosing and managing various bleeding disorders.

Purpose of the Study:

• To highlight the transformative potential of flow cytometry in platelet function testing.
• To emphasize its advantages over conventional methods like light transmission aggregometry.
• To discuss the integration of flow cytometry data into clinical practice for improved patient outcomes.

Main Methods:

• Utilizing flow cytometry for multiparameter analysis of whole blood samples.
• Employing a carefully selected panel of validated markers to assess platelet activation, secretion, and procoagulant function.
• Integrating flow cytometry data with clinical context and complementary laboratory findings.

Main Results:

• Flow cytometry demonstrates high sensitivity and requires low sample volumes.
• It is compatible with challenging specimens, including thrombocytopenic and pediatric samples.
• Multiparameter analysis provides detailed characterization of platelet functions.

Conclusions:

• Flow cytometry offers a more accessible, practical, and widely implementable approach to platelet testing.
• Validated marker panels combined with clinical context yield actionable diagnostic and management information.
• This technology bridges the gap between specialized research assays and routine clinical practice.