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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
06:18

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Published on: April 26, 2019

Nanoluciferase-Based Reporters for Clostridioides difficile.

Jeroen Corver1, Ana M Oliveira Paiva1, Wiep Klaas Smits2

  • 1Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|May 7, 2026
PubMed
Summary
This summary is machine-generated.

Nanoluciferase (NanoLuc®) is a versatile reporter enzyme. This study details its use in Clostridioides difficile for promoter activity, protein topology, and protein interactions, offering high sensitivity in cellular assays.

Keywords:
Gene expressionLuciferaseReporter gene assay

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Microbiology

Background:

  • Nanoluciferase (NanoLuc®) is a small, bright reporter enzyme with broad applicability.
  • Its detection relies on the substrate furimazine, enabling highly sensitive assays.
  • NanoLuc® can be adapted for various cellular analyses.

Purpose of the Study:

  • To describe the application of codon-optimized nanoluciferase in Clostridioides difficile.
  • To utilize NanoLuc® as a reporter for promoter activity.
  • To assess protein topology and investigate protein-protein interactions using NanoLuc®.

Main Methods:

  • Utilizing codon-optimized nanoluciferase reporter gene.
  • Employing furimazine substrate for sensitive detection.
  • Adapting NanoLuc® for promoter activity assays, protein topology studies, and protein interaction investigations in C. difficile.

Main Results:

  • Demonstrated successful application of NanoLuc® in C. difficile.
  • Validated NanoLuc® for monitoring promoter activity with high sensitivity.
  • Showcased utility in assessing protein topology and interactions.

Conclusions:

  • Codon-optimized nanoluciferase is a powerful and versatile tool for C. difficile research.
  • NanoLuc® enables sensitive and adaptable investigations of gene expression, protein structure, and molecular interactions.
  • This reporter system significantly advances molecular studies in Clostridioides difficile.