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Nanoluciferase-Based Reporters for Clostridioides difficile

Jeroen Corver1, Ana M Oliveira Paiva1, Wiep Klaas Smits2

  • 1Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|May 7, 2026
PubMed
Abstract

Keywords:
Gene expressionLuciferaseReporter gene assay

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...