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Updated: May 19, 2026
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Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
Published on: March 14, 2022
Transcriptomic profiling for identifying differentially expressed genes in aneurysm.
G Shirley Lois1, Shanmugapriya Murugan1, Mohana Shanmugam Jaganathan1
1Department of Bioinformatics, Sri Ramachandra Faculty of Engineering and Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai, India.
Bioinformation
|May 18, 2026
Summary
Aneurysm progression involves significant molecular changes, particularly in lipid metabolism pathways. Key genes identified may offer new therapeutic targets for treating this dangerous arterial condition.
Area of Science:
- Molecular Biology
- Genomics
- Cardiovascular Research
Background:
- Aneurysm progression is linked to poorly understood molecular alterations.
- Transcriptomic analysis of aneurysms remains limited.
- Aneurysms are arterial bulges exceeding 50% of normal diameter.
Purpose of the Study:
- To investigate transcriptomic changes in aneurysm progression.
- To identify key genes and pathways involved in aneurysm development.
- To explore potential therapeutic targets for aneurysm treatment.
Main Methods:
- RNA sequencing (RNA-Seq) analysis of 14 samples (7 control, 7 aneurysm).
- Pathway analysis to identify biological processes.
- Protein-protein interaction (PPI) network analysis using STRING database.
Main Results:
- Significant shifts in lipid metabolism pathways were observed.
- Key differentially expressed hub genes identified: LIPE, SREBF1, SCARB2, LPL, PNPLA2, UCP1, CIDEC, DGAT2, CIDEA, FABP4.
- These genes encode proteins involved in crucial cellular functions.
Conclusions:
- Transcriptomic insights reveal major alterations in lipid metabolism during aneurysm progression.
- Identified hub genes represent potential molecular targets for aneurysm therapies.
- Further research into these targets could lead to novel interventions.
