Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrastructural Analysis of In Vitro Glycated Engineered Skin.

Journal of cosmetic dermatology·2026
Same author

Stannous fluoride protects gingival keratinocytes against infection and oxidative stress by <i>Porphyromonas gingivalis</i> outer membrane vesicles.

Frontiers in dental medicine·2025
Same author

Stannous fluoride forms aggregates between outer and inner membranes leading to membrane rupture of <i>Porphyromonas gingivalis</i> and <i>Prevotella pallens</i>.

Frontiers in oral health·2024
Same author

Development of <i>in vitro</i> methods to model the impact of vaginal lactobacilli on <i>Staphylococcus aureus</i> biofilm formation on menstrual cups as well as validation of recommended cleaning directions.

Frontiers in reproductive health·2023
Same author

Multimodal Evidence of Mesostructured Calcium Fatty Acid Deposits in Human Hair and Their Role on Hair Properties.

ACS applied bio materials·2022
Same author

Nanoscale Mass Spectrometry Multimodal Imaging <i>via</i> Tip-Enhanced Photothermal Desorption.

ACS nano·2020
Same journal

An Automated Atom Probe Tomography Cluster Detection Approach Using Transfer Learning.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Correlative Light and Electron Microscopy Visualization of Helicobacter pylori in Human Saliva.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Integrating Morpho-Anatomy and Histochemistry to Characterize Native Brazilian Eugenia Species.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Postnatal Developmental Expression and Localization of Water Channel Proteins in the Rat Ovary.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
See all related articles

Related Experiment Video

Updated: May 28, 2026

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
08:29

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells

Published on: October 17, 2025

Optimized Broad Ion-Beam Workflow for Cross-Sectioning Multilayer Polymer Structures.

Seyed Aref Golsorkhi1,2, Vighter Iberi2, Marc Mamak2

  • 1Department of Mechanical and Materials Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

Optimizing broad ion milling of polymer laminates requires careful control of voltage and stage motion. Specific parameters minimize defects like curtaining and redeposition, ensuring structural fidelity for multilayer materials.

Keywords:
broad ion beamcross sectionion millingmicroscopypolymers

More Related Videos

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy
11:03

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Published on: July 14, 2022

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

Related Experiment Videos

Last Updated: May 28, 2026

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
08:29

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells

Published on: October 17, 2025

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy
11:03

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Published on: July 14, 2022

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

Area of Science:

  • Materials Science
  • Surface Science
  • Analytical Chemistry

Background:

  • Broad ion milling of multilayer polymer laminates faces challenges like curtaining, redeposition, and thermal smear.
  • These defects compromise the structural integrity at interfaces within the material.

Purpose of the Study:

  • To systematically investigate the effects of key parameters on ion milling outcomes for polymer laminates.
  • To identify optimal milling conditions that minimize defects and preserve structural fidelity.

Main Methods:

  • Systematic mapping of accelerating voltage, stage kinematics (oscillation), beam entry direction, and temperature.
  • Utilizing Energy Dispersive X-ray Spectroscopy (EDS) for compositional analysis at various magnifications.
  • Validation of optimized methods on a second, distinct polymer laminate.

Main Results:

  • An optimal operating point near 3 kV for 4 hours was identified, yielding minimal and correctable curtaining.
  • Superposing oscillatory stage motion significantly suppressed curtaining and redeposition.
  • Milling from the hard side established a stable reference plane, preventing shadowing and long curtains.
  • Room temperature milling with a brief flat-milling finish improved planarity.
  • EDS analysis revealed apparent composition is dictated by exposed band coverage, not inherent chemistry.

Conclusions:

  • Precise control over ion milling parameters is crucial for achieving high-fidelity cross-sections of multilayer polymer laminates.
  • Optimized milling strategies, including oscillatory motion and specific voltage/temperature settings, effectively mitigate common defects.
  • The findings are transferable to different laminate compositions and thicknesses, offering a robust method for materials analysis.