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Related Experiment Video

Updated: Jun 3, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

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Published on: November 8, 2015

Impact of Different Pre-Dilution Methods on Routine Blood Test Results: A Study With Sysmex XN-1000.

Lingsong Wu¹, Mengzhe Qin², Zhe Xing¹

¹Department of Medical Laboratory, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Journal of Clinical Laboratory Analysis

|June 2, 2026

Summary

This summary is machine-generated.

The wet-tip wiping method demonstrated superior analytical performance for pre-dilution testing on the Sysmex XN-1000 analyzer. This manual procedure is recommended for low-volume specimens, offering optimal results compared to other methods.

Keywords:

Bland–Altman analysis Sysmex XN‐1000 blood routine test pre‐dilution methods wet‐tip wiping

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Last Updated: Jun 3, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

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Published on: November 8, 2015

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

Area of Science:

Clinical laboratory science
Hematology automation
Diagnostic testing

Background:

Manual pre-dilution is often necessary for analyzing low-volume blood samples.
Evaluating different pre-dilution techniques is crucial for accurate automated hematology analysis.
The Sysmex XN-1000 analyzer offers automated undiluted testing modes.

Purpose of the Study:

To compare three manual pre-dilution methods against the automated undiluted mode on the Sysmex XN-1000.
To determine the optimal standard operating procedure for pre-dilution testing.
To assess the analytical performance of different pre-dilution techniques for key hematological parameters.

Main Methods:

Sixty whole-blood specimens were tested using automated undiluted mode and three manual pre-dilution methods (direct mixing, dry-tip wiping, wet-tip wiping).
Pre-dilution was performed at a 1:7 ratio using calibrated pipettes and Sysmex DCL diluent.
Hematological parameters (WBC, RBC, HGB, PLT, HCT) were analyzed, with results compared using statistical tests and bias analysis against CLIA criteria.

Main Results:

No significant differences were observed for WBC, RBC, HGB, and PLT between manual methods and the undiluted mode (p > 0.05).
The wet-tip wiping method showed the best overall agreement with the undiluted mode.
Only dry-tip and wet-tip wiping methods met allowable bias limits for all five parameters.

Conclusions:

The wet-tip wiping method offers the best analytical performance for pre-dilution on the Sysmex XN-1000.
This method is recommended for low-volume specimens requiring pre-dilution on this platform.
Optimized manual pre-dilution procedures ensure accurate results in automated hematology.