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Updated: Jun 10, 2026
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
A Standardized Metabolomics Protocol For Analyzing Exercise-Induced Metabolic Shifts In Elite Boxers By Liquid
Peng Sun1, Dong Zhang2, Qinyi Chen1
1Anhui Institute of Sports Science and Technology.
Journal of Visualized Experiments : Jove
|June 8, 2026
Summary
Elite boxing rapidly alters metabolism, impacting glycolysis and sulfur pathways. A new metabolomics workflow accurately captures these exercise-induced changes in elite boxers for better recovery insights.
Area of Science:
- Exercise Physiology
- Metabolomics
- Biochemistry
Background:
- Elite boxing causes rapid metabolic shifts.
- Conventional physiological measures don't fully capture these changes.
- Metabolomics offers a deeper understanding of exercise physiology.
Purpose of the Study:
- To apply a standardized untargeted serum metabolomics workflow.
- To profile metabolic changes in elite boxers after acute sparring.
- To identify key metabolites associated with immediate and short-term recovery.
Main Methods:
- Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QTOF-MS/MS) for metabolite profiling.
- Standardized sample collection and pooled quality-control monitoring.
- Multivariate statistical analysis and pathway interpretation.
Main Results:
- Sparring altered metabolites involved in glycolysis and gluconeogenesis.
- Sulfur metabolism changes were observed at 24 hours post-sparring.
- Specific metabolites like phosphatidylinositol and thiosulfate distinguished recovery states.
Conclusions:
- The developed metabolomics workflow enables reproducible profiling of exercise-related metabolic changes.
- This protocol is suitable for small-cohort studies on acute exercise and recovery.
- Findings highlight the utility of metabolomics in elite athlete monitoring.
