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Updated: Jun 10, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Characterisation of the Novel HLA-G*01:54 Allele by Next-Generation Sequencing

Xuan You1, Fang Wang1, Qimin Wu1

  • 1HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, China.

HLA
|June 9, 2026
PubMed
Abstract

Keywords:
HLA‐G*01:54next‐generation sequencingnovel allele

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.