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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 13, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Protocol for assessing lysosomal ion channel function in mammalian cells using lysosomal patch-clamp technique.

Yayu Wang1,2, Lily Yeh Jan1,3

  • 1Department of Physiology, University of California at San Francisco; San Francisco, CA 94158, USA.

Biorxiv : the Preprint Server for Biology
|June 12, 2026
PubMed
Summary

This study details a method for whole-endolysosome patch-clamp recordings in mammalian cells. It enables electrophysiological studies of enlarged endolysosomes, crucial for understanding cellular processes.

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

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Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

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Published on: April 4, 2025

One-channel Cell-attached Patch-clamp Recording
13:07

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
15:28

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Area of Science:

  • Cell Biology
  • Electrophysiology
  • Membrane Biophysics

Background:

  • Endolysosomes are critical organelles involved in cellular waste degradation and recycling.
  • Understanding endolysosome function requires precise electrophysiological measurements.
  • Existing techniques may not adequately address the unique challenges of endolysosome electrophysiology.

Purpose of the Study:

  • To describe a detailed protocol for manual whole-endolysosome patch-clamp recordings.
  • To enable electrophysiological characterization of enlarged endolysosomes.
  • To provide a foundation for future studies on endolysosome ion channel activity.

Main Methods:

  • Manual whole-endolysosome patch-clamp recording technique.
  • Pharmacological or genetic enlargement of endolysosomes in cultured mammalian cells.
  • High-resistance pipette fabrication, mechanical isolation of vesicles, and giga-seal formation.

Main Results:

  • Successful establishment of the whole-endolysosome patch-clamp configuration.
  • Demonstration of a reliable method for recording from isolated endolysosomes.
  • The protocol allows for detailed analysis of ion transport across the endolysosomal membrane.

Conclusions:

  • This protocol provides a robust method for studying endolysosome electrophysiology.
  • Enables investigation of ion channel function and transport in endolysosomes.
  • Facilitates research into lysosomal storage diseases and other endolysosome-related disorders.