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Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

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Published on: February 16, 2020

Visualized DNA Aptamer-Functionalized Silver Nanoclusters against Tuberculosis.

Chen Shen¹, Mei Li¹, Yunze Tai¹

¹Department of Laboratory Medicine, Clinical Laboratory Medicine Research Center, Sichuan Clinical Research Center for Laboratory Medicine, Institution of Medical and Engineering Integration for Molecular Diagnosis, West China Hospital, Sichuan University, Chengdu 610041, China.

|June 26, 2026

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Researchers developed a novel fluorescent nanoprobe, BM2-AgNCs, for diagnosing tuberculosis (TB) by detecting lipoarabinomannan (LAM). This probe also demonstrated significant antibacterial activity against Mycobacterium tuberculosis (M.tb), offering a dual approach for TB management.

Area of Science:

Biomedical Engineering
Nanotechnology
Infectious Diseases

Background:

Tuberculosis (TB) remains a global health threat due to challenges in diagnosis and treatment.
Current diagnostic methods for Mycobacterium tuberculosis (M.tb) lack sensitivity and specificity.
Drug resistance in M.tb complicates treatment efficacy.

Purpose of the Study:

To develop a multifunctional fluorescent nanoprobe for TB diagnosis and treatment.
To create a sensitive biosensor for detecting lipoarabinomannan (LAM), a TB biomarker.
To evaluate the anti-M.tb activity and therapeutic potential of the developed nanoprobe.

Main Methods:

Functionalization of silver nanoclusters (AgNCs) with DNA aptamer BM2 to create BM2-AgNCs.
Development of a fluorescence-based biosensor utilizing BM2-AgNCs for LAM detection.

Keywords:

DNA aptamer diagnosis silver nanoclusters treatment tuberculosis

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Assessment of the antibacterial efficacy of BM2-AgNCs against M.tb in vitro.

Investigation of BM2-AgNCs' effect on macrophage polarization and phagolysosomal maturation.

Main Results:

BM2-AgNCs enabled efficient detection of LAM with fluorescence recovery upon target binding.
The nanoprobe demonstrated significant reduction (80%) in both intracellular and extracellular M.tb viability.
BM2-AgNCs induced M1 polarization in macrophages and promoted phagolysosomal maturation, enhancing therapeutic effects.
The nanoprobe's fluorescence properties were leveraged for tracking M.tb and visualizing therapeutic mechanisms.

Conclusions:

The developed BM2-AgNCs offer a promising integrated platform for sensitive TB diagnosis and effective treatment.
This nanotechnology-based approach addresses limitations of current TB diagnostics and therapeutics.
The study highlights the potential of fluorescent nanoprobes in understanding and combating infectious diseases like TB.