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Updated: Jul 13, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
Mass Spectrometry as a Tool for Metabolomics in Cancer
João S Patrício1, Ana C L Guerreiro2,3, Paulo A Gameiro1
1iNOVA4Health, NOVA Medical School | Faculdade de Ciências Médicas, NMS, FCM, Universidade NOVA de Lisboa, Lisboa, Portugal.
None:
Mass spectrometry (MS) is a highly sensitive and high-throughput analytical technology that has become central to investigating the metabolic alterations in cancer cells, enabling the discovery of diagnostic biomarkers and potential therapeutic targets. This chapter provides an overview of the key principles of MS-based metabolomics, covering the entire experimental workflow-from sample collection and preparation to data acquisition, preprocessing, normalization, statistical modeling, and pathway enrichment analysis. We also discuss current limitations and outline future directions to enhance data reproducibility, metabolite annotation, and clinical translation of MS-based results.
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