Related Experiment Video
Updated: Aug 5, 2026

04:40
Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth
Published on: March 27, 2026
Objective Image-Based Assessment of Tooth Translucency Changes Following Different Bleaching Protocols: A
Ruben Pereira1, João Silveira2, Susana Dias1
1Oral Biology and Biochemistry Research Group, Faculty of Dental Medicine, Universidade de Lisboa, 1600-277 Lisboa, Portugal.
Biomimetics (Basel, Switzerland)
|July 27, 2026
Summary
Tooth bleaching significantly increases tooth translucency, with at-home methods showing greater changes than in-office treatments. An image-based analysis confirmed these technique-dependent effects on translucency.
Area of Science:
- Dental biomaterials
- Optical properties of teeth
- Aesthetic dentistry
Background:
- Tooth bleaching is a common aesthetic dental procedure.
- Tooth translucency is an optical property crucial for biomimetic dentistry.
- Evaluating bleaching effects on translucency requires objective methods.
Purpose of the Study:
- To assess changes in tooth translucency after three different bleaching protocols.
- To validate an objective, image-based, and spatially resolved method for translucency assessment.
- To compare the impact of in-office versus at-home bleaching on tooth translucency.
Main Methods:
- Retrospective analysis of spectrophotometric images from a randomized clinical trial.
- Comparison of three bleaching systems: in-office 6% hydrogen peroxide, at-home 6% hydrogen peroxide, and at-home 16% carbamide peroxide.
- Image analysis using ImageJ to quantify translucency parameters (area, grey intensity, RGB-blue).
Main Results:
- All bleaching protocols significantly increased translucency parameters (p < 0.05).
- At-home techniques induced greater translucency changes than in-office treatment (p < 0.05).
- Increased translucency persisted at six months, with weak correlations to color changes.
Conclusions:
- An image-based method effectively detected technique-dependent changes in tooth translucency post-bleaching.
- Bleaching significantly alters tooth translucency, with variations based on the method used.
- The correlation between translucency changes and overall color outcomes is weak.
