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An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Published on: July 31, 2019
Effects of Compound Probiotic Fermented Feed on In Vitro Rumen Fermentation, In Situ Degradation, Rumen Microbiota
Haitao Hu1, Yuwa Cao1, Mei Tian1
1College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Metabolites
|July 27, 2026
Summary
Compound probiotic fermented feed (CPFF) significantly improved rumen fermentation, feed degradation, and growth performance in beef cattle. The 4% CPFF supplementation optimized microbial communities and metabolic profiles, leading to increased average daily gain.
Area of Science:
- Rumen microbiology and fermentation
- Animal nutrition and feed science
- Livestock production and performance
Background:
- Compound probiotic fermented feed (CPFF) utilizes Lactobacillus plantarum, Bacillus subtilis, yeast, and Aspergillus niger.
- Evaluating CPFF's impact on ruminant digestion and growth is crucial for optimizing beef cattle production.
Purpose of the Study:
- To assess the effects of CPFF on in vitro rumen fermentation parameters.
- To evaluate CPFF's influence on in situ feed degradation characteristics.
- To determine the impact of CPFF supplementation on the growth performance of beef cattle.
Main Methods:
- In vitro fermentation trials with varying CPFF inclusion levels (2%, 4%, 8%).
- In situ degradation studies using neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude protein (CP).
- Rumen fluid analysis via 16S rRNA sequencing and untargeted metabolomics.
- Growth performance assessment in Angus growing cattle fed a diet supplemented with 4% CPFF.
Main Results:
- 4% CPFF supplementation significantly increased ammonia-nitrogen (NH3-N) and microbial crude protein (MCP) production.
- CPFF altered volatile fatty acid profiles, increasing acetate and butyrate while decreasing propionate.
- In situ trials showed enhanced NDF, ADF, and CP degradation rates with 4% CPFF.
- Microbial analysis revealed increased abundance of beneficial bacteria (e.g., Rikenellaceae_RC9_gut_group) and altered metabolic pathways.
- Beef cattle supplemented with 4% CPFF exhibited significantly higher average daily gain (ADG) and average daily feed intake (ADFI).
Conclusions:
- CPFF effectively enhances rumen fermentation, microbial community structure, and metabolic function in beef cattle.
- Supplementation with 4% CPFF optimizes feed utilization and significantly improves growth performance (ADG, ADFI).
- CPFF shows promise as a feed additive for improving beef cattle production efficiency.
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