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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
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Reply to: Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

Roman Teo Oliynyk1,2, George M Church3,4

  • 1Department of Genetics, Harvard Medical School, Boston, MA, USA. roli573@aucklanduni.ac.nz.

Nature Protocols
|July 30, 2026
PubMed
Abstract

No abstract available in PubMed .

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Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
PCR01:32

PCR

Overview
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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