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Updated: Aug 7, 2026
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Protocol for adoptive neutrophil transfer to study bone marrow and peripheral blood hematopoietic stem cell kinetics
Carlotta Boscaro1, Gian Paolo Fadini1, Mattia Albiero1
1Department of Medicine, University of Padova, 35128 Padova, Italy; Veneto Institute of Molecular Medicine, 35129 Padova, Italy.
The homeostatic clearance of neutrophils regulates the kinetics of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow. We present a protocol for studying the kinetics of HSPCs in mice using adoptive neutrophil transfer. We describe steps for isolating neutrophils from the bone marrow and performing an intravenous injection. We detail procedures for bone marrow flushing, immunomagnetic selection, neutrophil labeling, and successful tail-vein injection. For complete information on the generation and use of this protocol, please refer to Rodella et al.1.
The homeostatic clearance of neutrophils regulates the kinetics of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow. We present a protocol for studying the kinetics of HSPCs in mice using adoptive neutrophil transfer. We describe steps for isolating neutrophils from the bone marrow and performing an intravenous injection. We detail procedures for bone marrow flushing, immunomagnetic selection, neutrophil labeling, and successful tail-vein injection. For complete information on the generation and use of this protocol, please refer to Rodella et al.1.
