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Protective Effects of Double-layered Multinucleated Microcapsules Containing Bifidobacterium adolescentis FS2-3

Jiayi Tang1, Henan Zhang1, Danli Yan1

  • 1College of Food Science, Liaoning Engineering Research Center of Food Fermentation Technology, Shenyang Key Laboratory of Microbial Fermentation Technology Innovation, Shenyang Agricultural University, National Agricultural Environmental Microbial Germplasm Resource Bank, Shenyang, PR China.

Probiotics and Antimicrobial Proteins
|August 6, 2026
PubMed

Insights

Probiotic microcapsules enhanced Bifidobacterium adolescentis survival and reduced gut inflammation in mice. These microcapsules offer a promising strategy for treating bacterial enteritis by modulating gut microbiota and immune responses.

Area of Science:

  • Microbiology and Immunology
  • Gastroenterology
  • Biotechnology

Background:

  • Bifidobacterium adolescentis (B. adolescentis) is a key gut probiotic with known immune benefits.
  • The therapeutic potential of B. adolescentis against specific pathogenic bacteria and its efficacy in enteritis models require further investigation.
  • Improving probiotic intestinal colonization is crucial for enhancing their therapeutic effects.

Purpose of the Study:

  • To assess the inhibitory effects of B. adolescentis FS2-3 against common foodborne pathogens.
  • To develop and evaluate double-layered multinucleated microcapsules for enhanced B. adolescentis FS2-3 intestinal colonization and efficacy in treating bacterial enteritis.
  • To investigate the impact of probiotic microcapsules on gut microbiota composition, inflammation, and immune signaling pathways in vivo.

Main Methods:

  • In vitro testing of B. adolescentis FS2-3 against five pathogenic bacteria (Shigella dysenteriae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enteritidis, Campylobacter jejuni).
  • Construction of double-layered multinucleated microcapsules for B. adolescentis FS2-3 encapsulation.
  • In vivo assessment of probiotic microcapsules in a mouse model of bacterial enteritis, analyzing gut tissue damage, inflammation markers (TLR-4, IL-1β, NF-κB, TNF-α), and gut microbiota composition.

Main Results:

  • B. adolescentis FS2-3 demonstrated inhibitory activity against the tested pathogenic bacteria.
  • Encapsulation in microcapsules increased B. adolescentis FS2-3 survival by 5.76 times in vitro.
  • Probiotic microcapsules significantly reduced intestinal damage and inflammation in enteritis mice, particularly in Salmonella-infected models, by promoting beneficial bacteria and suppressing inflammatory pathways.

Conclusions:

  • Double-layered multinucleated microcapsules effectively enhance the survival and therapeutic potential of B. adolescentis FS2-3.
  • Probiotic microcapsules represent a viable strategy for managing bacterial enteritis by restoring gut microbiota balance and mitigating inflammation.
  • These findings highlight the application of advanced probiotic delivery systems for gastrointestinal health.

Keywords:
BifidobacteriumEnteritisGut microbiotaInflammationMicrocapsule

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