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Updated: Oct 25, 2026

Design and Synthesis of a Reconfigurable DNA Accordion Rack
Published on: August 15, 2018
The Development and Application of a ChemBead-Enabled On-DNA HTE Platform for Challenging C-N Couplings
Ana L Aguirre1, Amanda W Dombrowski1, Noah P Tu1
1AbbVie, Inc. , 1 N Waukegan Rd, North Chicago, Illinois60064, United States.
Abstract:
In this work, we present the creation and implementation of a ChemBead-enabled, on-DNA high-throughput experimentation (HTE) platform to identify more general and mild reaction conditions for challenging C-N couplings. The platform was developed by adapting our small-molecule system to address the inherent challenges of on-DNA chemistry. The results generated by the platform were compared to the standard operating procedure for DNA-encoded library (DEL) production to ensure the translation of any promising conditions. The platform was used to discover a condition that has not been previously reported for on-DNA C-N coupling. This condition is best suited for secondary amines and select primary amines on short DNA substrates; primary aliphatic amines on elongated DNA-tagged aryl halide conjugates remain a challenge. This newly identified condition can be applied to DNA-encoded library constructions.
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