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Updated: Aug 9, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
Separation of B and C type virions by centrifugation in gentle density gradients
Abstract:
Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm(3) when sucrose was used and from 1.12 to 1.28 g/cm(3) with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm(3), respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm(3) and with the C particle density little different from its value in sucrose.
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