[Effect of photooxidation on the immunological properties of immunoglobulins]

Photooxidation of anti-HSA antibodies, isolated by affinity chromatography, was carried out using methylene blue as a photosensitizing agent. Activity of anti-HSA antibody as well as an absorption maximum at 280 nM pH 7.0 disappeared due to photooxidation. Incubation of antibodies with methylene blue in the darkness was ineffective. Antigenic properties of human IgG were unaltered after photooxidation under the same conditions but the absorption maximum of the immunoglobulin at 280 nM disappeared.