Image analysis of FITC-immunofluorescence histochemistry in perivascular substance P-positive nerves

The ability of image analysis to quantify nerves labelled by FITC-immunofluorescence histochemistry has been tested on whole-mount stretch preparations of perivascular substance P-positive nerves. It has been shown that image analysis can make consistent measurements of nerves demonstrated by immunohistochemistry despite the fading of FITC-fluorescence which occurs during storage and on exposure to ultraviolet light.