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Contraction waves in lymphocyte locomotion.

W S Haston, J M Shields

    Journal of Cell Science
    |June 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    The constriction ring in white blood cells (leukocytes) generates significant locomotive force. Waves of contraction within this ring propel cell movement, especially when attached to external structures like collagen.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Immunology

    Background:

    • Leukocyte (white blood cell) motility is crucial for immune responses.
    • The precise mechanisms generating locomotive force in leukocytes remain incompletely understood.
    • The constriction ring is a notable feature of motile leukocytes.

    Purpose of the Study:

    • To investigate the role of the constriction ring as a source of locomotive force in leukocytes.
    • To analyze the dynamics of the constriction ring during cell movement.
    • To determine the relationship between constriction ring activity and cell polarity.

    Main Methods:

    • Time-lapse microscopy of lymphocytes in suspension and collagen gels.
    • Analysis of constriction ring morphology and movement.

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  • Transmission electron microscopy (TEM) for ultrastructural analysis.
  • NBD-Phallacidin staining to visualize F-actin distribution.

    • Main Results:

    • The constriction ring is the visible manifestation of an antero-posteriorly moving wave of circular contraction.
    • In suspension, the wave moves, but the cell does not; in collagen, the wave is stationary, and the cell moves through it.
    • Cell attachment to collagen via blebs is necessary for longitudinal contraction and movement.
    • F-actin is evenly distributed; cell polarity is established by organelle distribution but can be reversed by contraction waves.

    Conclusions:

    • The constriction ring is a major generator of locomotive force in leukocytes.
    • Cellular attachment to the extracellular matrix is essential for directed cell migration.
    • Contraction waves can independently alter cell polarity and drive movement.