[Comparison of a few methods for detecting antibodies to the bovine leukemia virus]

A comparative analysis of the results of bovine sera examinations by three methods for the detection of antibody to bovine leukemia virus (BLV): VSV BLV pseudotype neutralization test (PTNT), gel immunodiffusion using BLV glycoprotein antigen (GIDgp) and BLV 125I-p24 radioimmunoprecipitation (RIPp24), was performed. The results of all the three methods were in good correlation. The PTNT sensitivity was shown to exceed essentially all the other methods of BLV antibody detection. There was a definite correlation between the serum titer in PTNT and the possibility of antibody detection in RIPp24 and GIDgp. The potentials of these methods for diagnosis of BLV infection in animals are discussed.