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A role for fibronectin in cell sorting.

P B Armstrong, M T Armstrong

    Journal of Cell Science
    |July 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Embryonic chick heart mesenchymal cells (Mes) drive tissue self-organization by secreting fibronectin. This matrix formation allows Mes to cover and exclude myocytes (My), organizing developing heart tissue.

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    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Tissue Engineering

    Background:

    • Investigating embryonic morphogenesis requires understanding cell movement in organ culture.
    • Previous studies suggest mesenchymal cells (Mes) dominate associative behavior in chick heart ventricle tissue due to fibronectin matrix production.

    Purpose of the Study:

    • To experimentally investigate the hypothesis that mesenchymal cells control chick heart tissue organization through fibronectin matrix deposition.
    • To explore predictions regarding cell sorting and tissue patterning based on fibronectin availability.

    Main Methods:

    • Organ culture of paired embryonic chick heart ventricle aggregates.
    • Analysis of cell sorting and tissue interactions based on fibronectin matrix formation.
    • Experimental investigation of factors influencing mesenchymal cell behavior.

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    Main Results:

    • Mesenchymal cells (Mes) establish a fibronectin-rich pericellular matrix, dominating tissue interactions.
    • Mes cells sort to the surface of myocyte (My) aggregates, facilitated by serum-derived factors.
    • Fibronectin deposition increases Mes cohesiveness, enabling them to exclude myocytes and form pure Mes tissue.

    Conclusions:

    • Mesenchymal cell-driven fibronectin matrix formation is a key mechanism in embryonic heart tissue self-organization.
    • The distribution of a serum factor limits fibronectin deposition to superficial Mes cells, initiating tissue patterning.
    • This process explains how mesenchymal cells establish pure tissue domains within developing heart structures.