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Priming against stimulator-derived alloantigens bound onto MLC-activated blasts
Scandinavian Journal of Immunology
|January 1, 1980
Summary
This study shows that the immune system can be sensitized against foreign antigens transferred between cells. This specific immune response can be blocked by treating the cells with trypsin.
Area of Science:
- Immunology
- Cell Biology
Background:
- Antigen transfer between stimulator and responder cells is a known phenomenon.
- The ability to prime the immune system against transferred antigens remains an area of investigation.
Purpose of the Study:
- To investigate the possibility of immune sensitization against stimulator-derived alloantigens bound to mixed lymphocyte culture (MLC)-activated blasts.
- To determine if this sensitization could be specifically blocked.
Main Methods:
- Generated blasts with bound stimulator-derived alloantigens in a primary MLC.
- Primed a new family member against these blasts.
- Restimulated with all family members after 10 days.
- Treated blast cells with trypsin to assess response abolition.
Main Results:
- Successfully sensitized the immune system against transferred alloantigens.
- Achieved specific restimulation, confirming sensitization.
- Demonstrated that trypsin treatment abolished the specific immune response.
Conclusions:
- It is possible to induce an immune response against transferred alloantigens on MLC-activated blasts.
- This specific immune sensitization can be abrogated by trypsin treatment of the antigen-bearing cells.