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Related Experiment Videos

Characterization of liposomes prepared using a microemulsifier.

E Mayhew, R Lazo, W J Vail

    Biochimica Et Biophysica Acta
    |August 22, 1984
    PubMed
    Summary

    A novel device enables continuous, large-scale liposome preparation with high encapsulation efficiency. These liposomes demonstrate low leakage of cytosine arabinoside (Ara-C), showing potential for diagnostics and therapeutics.

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    Area of Science:

    • Biotechnology
    • Materials Science
    • Pharmaceutical Sciences

    Background:

    • Liposomes are crucial for drug delivery and diagnostics.
    • Efficient and scalable liposome preparation methods are needed.
    • Controlling liposome size and stability is key for applications.

    Purpose of the Study:

    • To develop a new device for continuous, high-yield liposome preparation.
    • To characterize the size, encapsulation efficiency, and stability of liposomes produced.
    • To evaluate the potential of these liposomes in various applications.

    Main Methods:

    • Utilized a novel microemulsifier device for continuous liposome production.
    • Employed cytosine arabinoside as an aqueous space marker.
    • Analyzed liposome size via electron microscopy.

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  • Assessed drug leakage at different temperatures and in serum over 48 hours.

    • Main Results:

    • Achieved excellent aqueous space capture efficiency (~75% for cytosine arabinoside at 300 µmol/ml).
    • Successfully produced liposomes with diameters less than 0.1 micron through recycling.
    • Demonstrated low leakage (<1% at 4°C, <10% at 37°C with serum over 48h) for specific lipid compositions.

    Conclusions:

    • The new device offers a scalable and efficient method for liposome preparation.
    • Liposomes produced exhibit desirable characteristics for drug delivery and other applications.
    • These liposomes show promise for use in diagnostics, therapeutics, and model membrane studies.