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Lowry method of protein quantification: evidence for photosensitivity
Analytical Biochemistry
|August 1, 1984
Summary
The Lowry protein assay, a convenient method for protein determination, is sensitive to light. This photosensitivity can cause up to a 10% error in protein concentration measurements if not managed.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- The Folin Phenol method (Lowry assay) is widely used for protein quantification.
- Previous studies have noted its susceptibility to various interfering factors.
Purpose of the Study:
- To investigate potential interfering factors affecting the Lowry protein assay.
- To determine the impact of photosensitivity on protein concentration measurements.
Main Methods:
- The study involved performing the Lowry protein assay.
- Measurements were taken under varying light conditions to assess photosensitivity.
Main Results:
- The Lowry assay demonstrated significant photosensitivity.
- Discrepancies of up to 10% in protein concentration were observed due to light exposure.
Conclusions:
- The Lowry protein assay is photosensitive, impacting accuracy.
- Appropriate precautions against light exposure are necessary for reliable protein quantification using this method.