Species-specific detection of Mycoplasma hyorhinis using DNA probes

Two specific DNA fragments from a genomic library of Mycoplasma hyorhinis demonstrated species-specific Southern and dot hybridization using a panel of different mycoplasmas. These probes selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this mycoplasma species without prior purification of the organism.