High-performance liquid chromatography of cisplatin

Area of Science:

• Analytical Chemistry
• Chromatography

Background:

• Cisplatin is a crucial chemotherapy drug with a need for accurate quantification in biological samples.
• Traditional chromatographic methods exhibit limitations in retaining and analyzing cisplatin effectively.

Purpose of the Study:

• To investigate the retention behavior of cisplatin on various stationary phases.
• To develop an optimized high-performance liquid chromatography (HPLC) method for cisplatin determination in urine.

Main Methods:

• Investigated cisplatin retention using aqueous mobile phases with electrolytes and methanol.
• Evaluated reverse-phase, silica, chemically bonded anion exchangers, and solvent-generated anion exchangers.
• Utilized column switching and off-line atomic absorption for detection.

Main Results:

• Cisplatin showed poor retention on reverse-phase and silica columns.
• Satisfactory retention was achieved on chemically bonded and solvent-generated anion exchangers.
• Solvent-generated anion exchangers demonstrated superior peak shape, column efficiency, and stability compared to chemically bonded systems.

Conclusions:

• Ion-dipole interactions and solvophobic theory explain cisplatin retention on positively charged phases.
• Solvent-generated anion exchange HPLC is a robust and advantageous method for cisplatin analysis.
• The developed method is applicable for determining cisplatin in urine samples.