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Proline and hepatic lipogenesis.

M C Sugden, D I Watts, P S West

    Biochimica Et Biophysica Acta
    |April 24, 1984
    PubMed
    Summary
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    Proline and lactate stimulate lipogenesis (fat production) in rat liver cells. However, proline

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Metabolic Research

    Background:

    • Lipogenesis, the process of fatty acid synthesis, is crucial for energy storage.
    • Lactate is a known precursor for lipogenesis.
    • The role of proline in lipogenesis requires further investigation.

    Purpose of the Study:

    • To investigate the effects of proline on lipogenesis in isolated rat hepatocytes.
    • To compare proline's lipogenic effects with those of lactate.
    • To elucidate the metabolic pathways involved in proline-stimulated lipogenesis.

    Main Methods:

    • Isolated rat hepatocytes were used to measure lipogenesis via 3H2O and 14C incorporation.
    • Experiments involved hepatocytes from fed rats (glycogen-depleted) and starved rats.

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  • Inhibitors of ATP-citrate lyase ((-)-hydroxycitrate) and phosphoenolpyruvate carboxykinase (3-mercaptopicolinate) were employed.

    • Main Results:

    • Both proline and lactate increased lipogenesis in hepatocytes from fed, glycogen-depleted rats.
    • Proline did not stimulate lipogenesis in hepatocytes from starved rats, unlike lactate.
    • (-)-Hydroxycitrate partially inhibited lactate-induced lipogenesis but completely inhibited proline-induced lipogenesis.
    • 3-Mercaptopicolinate did not inhibit proline- or lactate-induced lipogenesis.

    Conclusions:

    • Proline's lipogenic mechanism differs from lactate's, particularly in starved rat hepatocytes.
    • ATP-citrate lyase appears to be a key enzyme in proline-stimulated lipogenesis.
    • The findings highlight distinct roles for proline and lactate in hepatic lipogenesis and provide insights into regulatory pathways.