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Manganese-dependent cell-substratum adhesion.

F Grinnell

    Journal of Cell Science
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Manganese (Mn) is crucial for baby hamster kidney cell adhesion and spreading on various surfaces, even without typical adhesion factors. This Mn-dependent cell adhesion is specific and requires continuous Mn presence for cell attachment.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Biophysics

    Background:

    • Cell adhesion is critical for tissue development and function.
    • Adhesion is typically mediated by specific cell surface receptors binding to extracellular matrix proteins or other ligands.
    • The role of divalent cations, particularly manganese (Mn), in cell adhesion independent of known adhesion factors requires further investigation.

    Purpose of the Study:

    • To investigate the role of manganese in baby hamster kidney (BHK) cell adhesion and spreading.
    • To determine if manganese can mediate cell adhesion in the absence of traditional adhesion factors like fibronectin or lectins.
    • To characterize the nature of manganese-dependent cell adhesion and its requirements.

    Main Methods:

    • Culturing BHK cells in media supplemented with manganese (Mn) or other divalent cations (Mg, Ca, Fe, Co, Ni).

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  • Assessing cell attachment and spreading on various substrata, including protein-coated surfaces and collagen films.
  • Employing proteolytic treatments (trypsin, Pronase) and inhibitory antibodies to probe cell surface interactions.
  • Performing experiments involving the continuous presence or removal of Mn during cell adhesion and spreading.

    • Main Results:

    • BHK cells demonstrated significant attachment and spreading on diverse substrata in the presence of Mn, independent of added fibronectin or lectins.
    • Mn-dependent adhesion was specific, as other divalent cations could not substitute for Mn.
    • Proteolytic treatment of cell surface sites and inhibitory antibodies targeting ligand-mediated adhesion also affected Mn-dependent adhesion.
    • Continuous presence of Mn was essential for maintaining cell adhesion and spreading; removal led to cell detachment.

    Conclusions:

    • Manganese plays a critical and specific role in mediating baby hamster kidney cell adhesion and spreading.
    • This manganese-dependent adhesion mechanism appears distinct from classical fibronectin or lectin-mediated pathways.
    • The findings suggest the involvement of specific cell surface sites and potentially novel adhesion pathways regulated by manganese.