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[D-mannose-resistant hemagglutination in opportunistic enterobacteria in infantile diarrhea]
Abstract:
D-mannose-resistant hemagglutination with human and bovine red blood cells was determined in 284 cultures of opportunistis enterobacteria isolated from 99 patients with acute intestinal infections and in 55 cultures isolated from 27 practically healthy children aged up to 1 year. In 16 sick children (16.16%) opportunistic enterobacteria, positive when tested in D-mannose-resistant hemagglutination, were detected. In no case were such cultures isolated from practically healthy children. In 9 out of 12 species of opportunistic bacteria belonging to the family Enterobacteriaceae strains showing the positive reaction in the D-mannose-resistant hemagglutination test were found.
Insights
Opportunistic enterobacteria exhibiting D-mannose-resistant hemagglutination were identified in 16.16% of sick children with acute intestinal infections. These bacteria were not found in healthy children, suggesting a role in pediatric intestinal infections.
Area of Science:
- Microbiology
- Pediatric Infectious Diseases
- Bacteriology
Background:
- Opportunistic enterobacteria are common causes of intestinal infections in children.
- Hemagglutination is a bacterial virulence factor that can be assessed using D-mannose-resistant assays.
- The role of specific opportunistic enterobacteria strains in pediatric acute intestinal infections requires further investigation.
Purpose of the Study:
- To investigate the prevalence of D-mannose-resistant hemagglutination in opportunistic enterobacteria isolated from children.
- To determine if D-mannose-resistant hemagglutination is associated with acute intestinal infections in pediatric patients.
- To identify bacterial species within the Enterobacteriaceae family exhibiting this characteristic.
Main Methods:
- Collected 284 opportunistic enterobacteria cultures from 99 patients with acute intestinal infections.
- Collected 55 opportunistic enterobacteria cultures from 27 healthy children (aged ≤1 year).
- Performed D-mannose-resistant hemagglutination tests using human and bovine red blood cells.
Main Results:
- D-mannose-resistant hemagglutination was detected in opportunistic enterobacteria from 16.16% (16/99) of sick children.
- No D-mannose-resistant hemagglutination-positive cultures were isolated from any of the healthy children.
- Positive D-mannose-resistant hemagglutination reactions were found in strains across 9 out of 12 tested opportunistic bacterial species from the Enterobacteriaceae family.
Conclusions:
- D-mannose-resistant hemagglutination is a significant characteristic observed in opportunistic enterobacteria from children with acute intestinal infections.
- The absence of these bacteria in healthy controls suggests a potential etiological role in pediatric intestinal disease.
- Further research into these specific bacterial strains could elucidate their pathogenic mechanisms in acute intestinal infections.